Thermo Fisher Scientific Phospho-p70 S6 Kinase (Ser411) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phosphorylated p70 S6 Kinase (Ser411). Validated for WB, IHC, and ICC/IF. Useful for studies of cell proliferation and signaling pathways. Supplied as unconjugated liquid, 1 mg/mL, stored at -20°C.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554339 |
Product Specific Information
- Suggested positive control for Western blot: MCF-7 cells
- Suggested positive control for IHC: Human breast carcinoma
- Suggested positive control for ICC/IF: MCF-7 cells
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with two non-identical catalytic domains that phosphorylate residues of the S6 ribosomal protein. It is mainly localized in the cytoplasm and plays a critical role in growth factor-regulated cell proliferation, motility, immune response, and tissue repair. Acting downstream of phosphoinositide (PI) 3-kinase, its main physiological target is the S6 ribosomal protein, which promotes protein synthesis. Overexpression and gene amplification of p70 S6 kinase are observed in certain breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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