Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), NovaFluor Red 755, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.4 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 786
• Functional Grade
• NovaFluor Red 700
• NovaFluor Yellow 690 / 700
• PE / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 780
• Custom conjugation available

Product Specific Information

Description:
The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a member of the B7 family (~40 kDa, Ig superfamily). It is expressed on most leukocytes (T, B, NK, DC) and acts as a ligand for PD-1. Interaction between PD-1 and PD-L1 (B7-H1) or PD-L2 (B7-DC) inhibits T and B cell responses. MIH5 is reported as a blocking antibody.

Applications Reported:

• Reported for use in flow cytometric analysis.

Applications Tested:

• Tested on mouse splenocytes for flow cytometry.
• Use ≤ 0.4 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
• Optimize antibody titration for best performance.

NovaFluor Dye Compatibility:

• Not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Use of CellBlox Blocking Buffer:

• Each NovaFluor conjugate ships with CellBlox Blocking Buffer.
• Add 5 µL per sample (regardless of number of NovaFluors).
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:

• Based on Phiton™ technology utilizing novel nucleic acid dye structures.
• Engineered for optimized spectral properties and reduced spillover.
• Excitation: 636 nm; Emission: 756 nm; Laser: 633–640 nm (Red).

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member binding to PD-1, which negatively regulates T-cell receptor (TCR) signaling. PD-L1 engagement decreases TCR-mediated proliferation and cytokine production. It is abundantly expressed on many murine and human cancers and upregulated by IFN-γ, suggesting a role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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