Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Blue 760, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Blue 760

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–737
• Brilliant Violet 480–711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510–800
• NovaFluor Red 700–755
• NovaFluor UV 765
• NovaFluor Violet 690, 800
• NovaFluor Yellow 590–810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR to induce phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for use at 5 µL (0.4 µg)/test
• Cell range: 10⁵–10⁸ cells/test

Important:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for optimal results.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance. Add 5 µL per sample to antibody cocktails or single-color controls.

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

Laser Compatibility: Excitation 492 nm; Emission 766 nm; Laser 488 nm (Blue)

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating proliferation and differentiation. Mutations in CD19 lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.

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