Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555
Alexa Fluor 555로 표지된 Donkey anti-Sheep IgG(H+L) 항체로, WB, IHC, ICC/IF에 적합합니다. 교차흡착 처리로 비특이적 결합을 최소화하며, 고순도의 액상 형태로 제공됩니다. Excitation/Emission은 553/568 nm이며, 4°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 555 |
| Excitation / Emission Max | 553 / 568 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535857 |
| Target | IgG |
| Cross Adsorption | Against mouse, rabbit, bovine and human sera and human IgG |
| Antibody Form | Whole Antibody |
Additional Information
- Product will be shipped at room temperature.
- Cross-adsorbed to minimize cross-reactivity with non-target species.
- Available in multiple Alexa Fluor conjugates (350, 488, 546, 568, 594, 633, 647, 680).
- Custom conjugation available upon request.
Target Information
Anti-Sheep secondary antibodies are affinity-purified with high specificity for ovine (sheep) immunoglobulins. These antibodies are used for detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. They are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified for specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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