Thermo Fisher Scientific Phospho-MDM2 (Thr218) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating the stability of p53. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting MDM2-mediated ubiquitination and degradation of p53. Phosphorylation also prevents MDM2 from binding to p19ARF, further increasing p53 degradation.

MDM2 negatively regulates p53 function by inhibiting its transactivation activity and promoting its degradation via E3 ubiquitin ligase activity. Overexpression of MDM2 can lead to inactivation of p53, reducing tumor suppressor function. MDM2 also plays key roles in apoptosis and the cell cycle and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer. Beyond p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing cell cycle, apoptosis, and tumorigenesis. More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.