Thermo Fisher Scientific Phospho-MDM2 (Thr218) Polyclonal Antibody
Phospho-MDM2 (Thr218) 특이적 폴리클로날 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능. Rabbit IgG 기반으로 Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human MDM2 around the phosphorylation site of Thr218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating the stability of p53. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting MDM2-mediated ubiquitination and degradation of p53. Phosphorylation also prevents MDM2 from binding to p19ARF, further increasing p53 degradation.
MDM2 negatively regulates p53 function by inhibiting its transactivation activity and promoting its degradation via E3 ubiquitin ligase activity. Overexpression of MDM2 can lead to inactivation of p53, reducing tumor suppressor function. MDM2 also plays key roles in apoptosis and the cell cycle and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer. Beyond p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing cell cycle, apoptosis, and tumorigenesis. More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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