Thermo Fisher Scientific BRF1 Polyclonal Antibody
BRF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, IP 등 다양한 응용에 적합하며, 고순도 액상 형태로 제공. 인체 BRF1(550-600) 영역을 면역원으로 사용하여 연구용으로 최적화됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 and 600 of human B-related factor 1 (BRF1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
RNA polymerase III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. To sustain rapid growth, cells must produce high levels of Pol III-transcribed RNA, requiring TFIIIB and TFIIIC2 transcription factor complexes.
TFIIIC2 complex consists of TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63, which are overexpressed in malignant cells such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be key in malignant cell deregulation.
The TFIIIB complex includes TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, whose expression is upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to bind and inactivate TFIIIB function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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