Thermo Fisher Scientific BRF1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

RNA polymerase III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. To sustain rapid growth, cells must produce high levels of Pol III-transcribed RNA, requiring TFIIIB and TFIIIC2 transcription factor complexes.
TFIIIC2 complex consists of TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63, which are overexpressed in malignant cells such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be key in malignant cell deregulation.
The TFIIIB complex includes TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, whose expression is upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to bind and inactivate TFIIIB function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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