Thermo Fisher Scientific GLRB Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunoprecipitation (IP)

• Tested Dilution: 5–10 µL/mg of lysate

Product Specifications

Target Information

In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential to voluntary motor control and reflex responses. Glycine binds to glycine receptors (GlyR) in the postsynaptic neuronal membranes. GlyR, gamma-aminobutyric acid, serotonin, and acetylcholine comprise an evolutionally conserved superfamily of ligand-gated ion channels. The pentameric subunit structure of GlyR consists of two types of glycosylated membrane proteins (alpha1 through alpha4 and beta) and an associated peripheral membrane protein, forming a chloride-selective ion channel. In humans, the composition of the pentamer changes from alpha2 subunits in the fetal CNS to alpha1 and beta subunits in the adult CNS. Fast potentiation of GlyR by intracellular Ca2+ in the brainstem and midbrain indicates an important role for Ca2+ in modulation of glycinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.