Thermo Fisher Scientific SPEN Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SPEN. Recombinant protein control fragment (Product #RP-91596). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPEN,uniProtId:Q96T58-1,ncbiNodeId:9606,antigenRange:139-284,antigenLength:3664,antigenImageFileName:PA5-53388_SPEN_Q96T58-1_Rabbit.svg,antigenImageFileNamePDP:PA5-53388_SPEN_Q96T58-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.10 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647841

Product Specific Information

Immunogen sequence: NRERAYEHSA YGHHERGTGG FDRTRHYDQD YYRDPRERTL QHGLYYASRS RSPNRFDAHD PRYEPRAREQ FTLPSVVHRD IYRDDITREV RGRRPERNYQ HSRSRSPHSS QSRNQSPQRL ASQASRPTRS PSGSGSRSRS SSSDSI

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.

Target Information

May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.