Thermo Fisher Scientific PFTK2 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:20–1:50

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
PRSLHVVWNR LGRVPEAEDL ASQMLKGFPR DRVSAQEALV HDYFSALPSQ LYQLPDEESL FTVSGVRLKP EMCDLLASYQ KGHHPA

Highest antigen sequence identity to the following orthologs:

• Mouse: 85%
• Rat: 86%

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this mechanism, protein kinases mediate most signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.

With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family is classified into eight major groups based on sequence comparisons of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) includes 50 kinases related to the MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs), are well characterized in the yeast pheromone response pathway.

Pheromones bind to Ste cell surface receptors and activate the yeast MAPK pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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