Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (BTN399)
Biotin(Vitamin B7/H)에 특이적인 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. Mouse IgG1, kappa 아이소타입이며, Protein A/G 정제. 연구용으로 안정적이며 다양한 시료 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| Immunomicroscopy (IM) | Assay-dependent |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BTN399 |
| Immunogen | Biotinylated sheep immunoglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is essential for all organisms and synthesized only by bacteria, yeasts, molds, algae, and certain plants.
Biotin participates in the incorporation of carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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