Thermo Fisher Scientific CDK1 Monoclonal Antibody (A17.1.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mg protein lysate

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Guinea pig, Human, Mouse, Rodent, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

A17.1.1

Immunogen

C-Terminal 2/3 of Xenopus CDC2 expressed in E. coli if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDK1,uniProtId:P35567-1,ncbiNodeId:8355,antigenRange:302,antigenLength:302,antigenImageFileName:MA5-11472_CDK1_P35567-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11472_CDK1_P35567-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979734

Product Specific Information

MA5-11472 targets Cdk1 in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Chicken, Guinea Pig, Human, mouse, Rodent, Rat, and Xenopus laevis samples.

The MA5-11472 immunogen is c-Terminal 2/3 of Xenopus CDC2 expressed in E. coli.

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.