Thermo Fisher Scientific GTF2B Monoclonal Antibody (OTI2F5), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:2,000

Product Specifications

Target Information

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment, and shuttle to the 26S proteasome is a vital means of regulating concentrations of key regulatory proteins by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps include activation of ubiquitin, ATP-dependent formation of a thioester bond between ubiquitin and enzyme E1, transfer to E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse this marking. Posttranslational tagging by ubiquitin is involved in diverse cellular processes such as the cell cycle, growth, differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, transcription regulation, transport, stress responses, immune response, and nervous system functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.