Thermo Fisher Scientific MBD1 Monoclonal Antibody (OTI1A1), TrueMAB
인간 MBD1 단백질(aa 222-556)을 항원으로 제작된 마우스 단클론 항체로, Western blot에 최적화됨. 비접합형 액상 형태이며, 1 mg/mL 농도로 PBS/BSA/glycerol buffer에 보존. 유전자 발현 조절 연구용으로 적합.
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Applications
- Western Blot (WB): Tested dilution 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 222–556 of human MBD1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It functions as a transcriptional repressor that binds CpG islands in promoters where DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG produced by DNA damage via methylmethanesulfonate (MMS). MBD1 recruits AFT7IP, which in turn recruits histone methyltransferase SETDB1, contributing to gene silencing. Isoforms 1 and 2 can also repress transcription from unmethylated promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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