
Thermo Fisher Scientific Phospho Enol Pyruvate Carboxylase Polyclonal Antibody, HRP
식물 유래 Phospho Enol Pyruvate Carboxylase를 인식하는 HRP 결합 폴리클로날 항체입니다. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합하며, 고순도 Ion-exchange chromatography로 정제되었습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho Enol Pyruvate Carboxylase Polyclonal Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Plant |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-enol-pyruvate Carboxylase [Maize Leaves] |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
Cross-reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but has not been specifically determined.
Target Information
Phosphoenolpyruvate Carboxylase catalyzes the carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
The enzyme is regulated by light-reversible phosphorylation and is involved in the C3 acid pathway, a key step in photosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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