Thermo Fisher Scientific PCMT1 Polyclonal Antibody
PCMT1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫트 반응성이 있으며, 고순도 Affinity Chromatography로 정제된 액상 항체입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 62–286 of human PCMT1 (NP_001238978.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.11 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855493 |
Product Specific Information
Immunogen sequence:
KSGGASHSEL IHNLRKNGII KTDKVFEVML ATDRSHYAKC NPYMDSPQSI GFQATISAPH MHAYALELLF DQLHEGAKAL DVGSGSGILT ACFARMVGCT GKVIGIDHIK ELVDDSVNNV RKDDPTLLSS GRVQLVVGDG RMGYAEEAPY DAIHVGAAAP VVPQALIDQL KPGGRLILPV GPAGGNQMLE QYDKLQDGSI KMKPLMGVIY VPLTDKEKQW SRDEL
Target Information
Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells.
The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues.
These methyl-accepting residues result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins.
PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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