Thermo Fisher Scientific SMAD7 Polyclonal Antibody
SMAD7 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 인간 시료에 반응하며, rat에서도 예측 반응. 고순도 정제 및 안정한 PBS buffer 포뮬레이션으로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 195–224 of human SMAD7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion, Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at –20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2543412 |
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Target Information
SMADs are members of the MAD-related family of intracellular proteins that play essential roles in the signaling pathways of serine/threonine kinase receptors of the TGF-β superfamily.
They are categorized into:
- R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8, SMAD9
- Co-SMAD: SMAD4
- I-SMADs: SMAD6, SMAD7
SMAD1, SMAD5, SMAD8, and SMAD9 share high sequence homology, and antibodies recognizing common sequences are available. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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