Thermo Fisher Scientific ERI1 Monoclonal Antibody (OTI1B3), TrueMAB

Applications

• Western Blot (WB): 1:500–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

ERI1 is an RNA exonuclease that binds to the 3′-end of histone mRNAs and degrades them, playing an essential role in histone mRNA decay after replication.
It requires the 2′ and 3′-hydroxyl groups at the last nucleotide of the histone 3′-end for efficient degradation.
ERI1 can also degrade 3′-overhangs of siRNAs in vitro, suggesting a role in RNA interference regulation.
It binds specifically to the 5′-ACCCA-3′ sequence within the stem-loop structure and interacts with other mRNAs and 5.8S rRNA.
Essential for 5.8S rRNA 3′-end processing and binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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