Humabiologics HumaMatrix- Native Human-Derived ECM, Lyophilized (HML)

HumaMatrix- Native Human-Derived ECM, Lyophilized (HML)

HumaMatrix is extracted from nutrient-rich human tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. HumaMatrix has undergone a proprietary manufacturing method to preserve native matrix proteins and growth factors. HumaMatrix is supplied lyophilized for cell culture & hydrogel applications and can be reconstituted for custom applications.

Product Description

HumaMatrix is one of the first commercially available native, human-derived extracellular matrix (ECM) products. Due to its human origin and minimal processing, HumaMatrix retains the native matrix proteins and growth factors found in human tissue including collagen, elastin, laminin, glycosaminoglycans and many other matrix proteins.

HumaMatrix is ideal for many applications including coating tissue culture surfaces to support cell attachment and growth or for making hydrogels.

Tissue Source

HumaMatrix is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue banks and organ procurement organizations (OPOs). Humabiologics strives to meet research needs by providing high quality biomaterials obtained from tissue partners who comply with requirements for transplantable human tissues under 21 CFR 1271 of the Food and Drug Administration (FDA).

Precautions and Disclaimer

HumaMatrix is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. HumaMatrix is for research use only and is not intended for human use, diagnosis, screening, household, food or other uses.

Storage/Reconstitution

HumaMatrix should be stored at -20 °C upon receipt. HumaMatrix is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf life of 6 months after opening.

HumaMatrix is soluble in 10-20 mM HCl with a final pH of 1.9-2.1. Reconstitution may take 3 hours at ambient temperature with vigorous shaking/vortexing or overnight at 4°C. Solution can be briefly centrifuged to remove bubbles. Do not let the temperature exceed 30 °C during reconstitution. After reconstitution, solution should be used immediately or aliquoted and frozen at -80 °C.

HumaMatrix-Coated Tissue Cultureware

Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications and cell types. Optimal ECM concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 - 100 µg/ml of ECM is used.

1. Reconstitute HumaMatrix in 10-20 mM HCl with a pH of 1.9-2.1 to a concentration of 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.

2. Mix by shaking or vortexing until the solution is homogeneous.

3. Aseptically add ECM solution to tissue culture surface to evenly cover entire surface.

4. Cover and incubate ECM coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.

5. Transfer ECM coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.

6. Rinse the coated culture surface with sterile PBS or culture medium to remove residual acid.

7. Use coated cultureware immediately or keep sterile and store at 2-10 °C.

Making 3D Hydrogels

Note: All procedures should be performed in a cold room or on ice. All reagents should be cooled prior to making the hydrogel. To prevent premature gelation, keep solutions between 2-10 °C. Concentrations will need to be tested to determine the optimal gel concentration for each application. HumaMatrix has been tested and found to form a gel at ≥ 3 mg/ml final concentration.

1. Reconstitute HumaMatrix in 10-20 mM HCl with a pH of 1.9-2.1. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.

2. Prepare 10x culture medium or 10x PBS with antibiotic and antimycotic.

3. Combine 9 volumes of chilled solution with 1 volume of chilled 10x concentrated medium or 10x PBS.

4. Gently swirl or pipette the mixture repeatedly.

5. Adjust the pH to 7.0-8.0, preferably around 7.4. Concentration and pH will affect the speed and strength of the final gel. Once pH has been adjusted, cells can be added to the solution if desired.

6. Incubate at 37 °C for 30-120 minutes for gel formation depending on final concentration.