Thermo Fisher Scientific SOD2 (MnSOD) Polyclonal Antibody
Thermo Fisher Scientific의 SOD2 (MnSOD) 폴리클로날 항체는 인간, 마우스, 랫트 시료에 반응하며 WB, IHC, ICC, ELISA에 사용 가능합니다. 고순도 친화 크로마토그래피 정제 및 -20°C 보관 조건으로 안정적입니다. 미토콘드리아 MnSOD 검출에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 25–222 of human SOD2 (NP_000627.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.57 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901192 |
Product Specific Information
Positive Samples: 293T, HepG2, A-549, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain, Rat heart
Immunogen Sequence:
KHSLPDLPYD YGALEPHINA QIMQLHHSKH HAAYVNNLNV TEEKYQEALA KGDVTAQIAL QPALKFNGGG HINHSIFWTN LSPNGGGEPK GELLEAIKRD FGSFDKFKEK LTAASVGVQG SGWGWLGFNK ERGHLQIAAC PNQDPLQGTT GLIPLLGIDV WEHAYYLQYK NVRPDYLKAI WNVINWENVT ERYMACKK
Target Information
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS).
SOD catalyzes the dismutation of superoxide radical anion (O₂⁻) to hydrogen peroxide, which is then converted to water and oxygen by glutathione peroxidase and catalase.
There are several classes of SOD:
- SOD1 (Cu,Zn-SOD): Cytosolic enzyme found in all eukaryotes (32 kDa homodimer).
- SOD2 (Mn-SOD): Mitochondrial enzyme (80 kDa tetramer) located in the mitochondrial matrix, near the respiratory chain.
- SOD3 (EC-SOD): Extracellular heparin-binding multimer expressed in lungs, vessels, and airways.
- SOD4 (CCS): Copper chaperone that delivers Cu to SOD1, activating it by cofactor insertion.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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