Thermo Fisher Scientific PCK1 Recombinant Rabbit Monoclonal Antibody (JU84-39)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU84-39

Immunogen

Synthetic peptide within Human PCK1 aa 26-75 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PCK1,uniProtId:P35558-1,ncbiNodeId:9606,antigenRange:26-75,antigenLength:622,antigenImageFileName:MA5-34631_PCK1_P35558-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34631_PCK1_P35558-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848539

Product Specific Information

Positive Control: 293T, HepG2, SH-SY5Y, human liver tissue, human kidney tissue.

Target Information

Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.