
Thermo Fisher Scientific COASY Monoclonal Antibody (OTI3B3), TrueMAB
COASY 단백질을 인식하는 마우스 단클론항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. HEK293T 세포에서 발현된 재조합 인간 COASY 단백질을 면역원으로 사용하였으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B3 |
| Immunogen | Full length human recombinant protein of human COASY produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, an additional desalting step is recommended (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes.
COASY is a bifunctional enzyme that catalyzes the last two steps in CoA synthesis.
In prokaryotes, these activities are performed by two separate enzymes: phosphopantetheine adenylyltransferase and dephospho-CoA kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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