
Thermo Fisher Scientific XPF Monoclonal Antibody (OTI2C2), TrueMAB
ERCC4(XPF) 단백질을 인식하는 Mouse monoclonal antibody로, WB, ICC/IF, Flow Cytometry에 사용 가능. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. DNA 손상 복구 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C2 |
| Immunogen | Full length human recombinant protein of human ERCC4 produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The ERCC1/XPF endonuclease complex is involved in DNA repair mechanisms, including:
- Nucleotide excision repair (NER) for UV-induced lesions and bulky adducts
- Recombination repair of interstrand crosslinks
In mammalian cells:
- XPG cleaves the DNA 3′ side of the lesion
- ERCC1/XPF complex cleaves the 5′ side
This complex likely also participates in processing recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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