Thermo Fisher Scientific Complement C3b Monoclonal Antibody (H11)

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL
• Publications: View 2 publications

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: 1:10
• Publications: View references

Product Specifications

Product Specific Information

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C or -20°C if preferred.
Mouse anti Human C3b antibody, clone H11 recognizes human C3b, part of the complement system.

Target Information

Inactive complement component 3b (iC3b) is a 42 kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated.

iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen-presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor, providing a link between the innate and adaptive immune responses. Additionally, iC3b acts as an opsonin recognized by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells.

iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosus and Rheumatoid Arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.