
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550
Goat anti-Rabbit IgG (H+L) DyLight 550은 Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합한 형광 표지 2차 항체입니다. DyLight 550 형광 염료로 높은 신호 강도와 낮은 배경을 제공합니다. Rabbit IgG에 특이적이며, 항원 친화 크로마토그래피로 정제되었습니다.
- 카탈로그번호
- 84541
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 4 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | DyLight™ 550 |
| Excitation/Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_10942173 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #84541 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. Cross-reactivity with immunoglobulins from other species may occur.
Store protected from light at 4°C until opened. To extend shelf life, add an equal volume of glycerol to achieve approximately 50% glycerol and store at -20°C.
DyLight 550 Amax = 562 nm; Emax = 576 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host animal with pooled rabbit immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- AP
- Biotin
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Request custom conjugation available
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