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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550

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Goat anti-Rabbit IgG (H+L) DyLight 550은 Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합한 형광 표지 2차 항체입니다. DyLight 550 형광 염료로 높은 신호 강도와 낮은 배경을 제공합니다. Rabbit IgG에 특이적이며, 항원 친화 크로마토그래피로 정제되었습니다.

카탈로그번호
84541
판매단위
pk
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마지막 업데이트 2025. 08. 02. 오후 03:46
Thermo Fisher Scientific 84541 Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550 1 mL pk판매 단위 pk ·
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326,500원VAT 포함 359,150원

Thermo Fisher Scientific · Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550

Applications and Tested Dilution

Application Tested Dilution
Western Blot (WB) 1:5,000–1:20,000
Immunohistochemistry (IHC) 1:50–1:2,000
Immunocytochemistry (ICC/IF) 4 µg/mL
Flow Cytometry (Flow) 1:25–1:100
Immunoprecipitation (IP) Assay-dependent

Product Specifications

Specification Description
Species Reactivity Rabbit
Host/Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Purified Rabbit IgG, whole molecule
Conjugate DyLight™ 550
Excitation/Emission Max 556 / 570 nm
Form Liquid
Concentration 1 mg/mL
Purification Antigen affinity chromatography
Storage Buffer PBS, pH 7.2, with 1% BSA
Contains 0.02% sodium azide
Storage Conditions 4°C
Shipping Conditions Ambient
RRID AB_10942173
Target IgG
Antibody Form Whole Antibody

Product Specific Information

Product #84541 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. Cross-reactivity with immunoglobulins from other species may occur.

Store protected from light at 4°C until opened. To extend shelf life, add an equal volume of glycerol to achieve approximately 50% glycerol and store at -20°C.
DyLight 550 Amax = 562 nm; Emax = 576 nm. Mole Dye/Mole Protein Ratio is lot-dependent.


Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host animal with pooled rabbit immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.


For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


Additional Formats

  • Alexa Fluor 488-10 nm colloidal gold
  • Alexa Fluor 488-5 nm colloidal gold
  • AP
  • Biotin
  • DyLight 350
  • DyLight 405
  • DyLight 488
  • DyLight 594
  • DyLight 633
  • DyLight 650
  • DyLight 680
  • DyLight 800 4X PEG
  • FITC
  • HRP
  • PE-Cyanine5.5
  • TRITC
  • WesternDot 625
  • Request custom conjugation available

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