Thermo Fisher Scientific Mouse GM-CSF Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 5 publications 5 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

-

View 969 publications 969 publications

Miscellaneous PubMed (Misc)

-

View 97 publications 97 publications

Product Specifications

Species

Mouse

Published species

Dog, Fish, Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Molecular weight

14.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.