Thermo Fisher Scientific GSPT1 Polyclonal Antibody
Thermo Fisher Scientific의 GSPT1 Polyclonal Antibody는 인간 GSPT1 단백질을 인식하는 토끼 유래 IgG 항체입니다. WB, IHC, ICC/IF에 사용 가능하며, 높은 특이성과 안정성을 제공합니다. 연구용으로 세포 성장 및 번역 종결 조절 연구에 적합합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–499 of Human GSPT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.46 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545732 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Predicted reactivity: Mouse (91%), Rat (93%), Chicken (81%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eRF3a (eukaryotic peptide chain release factor subunit 3a), also known as GSPT1 (G1 to S phase transition 1), is a 499 amino acid protein that belongs to the GTP-binding elongation factor family. It regulates cell growth via control of translation termination. Human eRF3a shares 94% sequence identity with its mouse counterpart, indicating conserved function. The gene encoding eRF3a maps to human chromosome 16, which contains over 900 genes (≈3% of the human genome). Mutations in the GAN gene on chromosome 16 may lead to giant axonal neuropathy, a nervous system disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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