Thermo Fisher Scientific NDFIP2 Polyclonal Antibody
Thermo Fisher Scientific의 NDFIP2 Polyclonal Antibody는 인간 NDFIP2 단백질을 인식하는 토끼 유래 IgG 항체입니다. Western blot, IHC(P), ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NDFIP2 (Product #RP-89600) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644532 |
Product Specific Information
Immunogen sequence:
AAAGATGSEE LPPGDRGCRN GGGRGPAATT SSTGVAVGAE HGEDSLSRKP DPEPGRMDHH QPGTGRYQVL LNEEDN
Antigen sequence identity:
- Mouse: 68%
- Rat: 74%
Target Information
NDFIP2 activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1, and WWP2, modulating the stability of their targets and influencing various cellular processes. It recruits ITCH, NEDD4, and SMURF2 to endosomal membranes, negatively regulates KCNH2 potassium channel activity, and may modulate EGFR signaling. Together with NDFIP1, it limits cytokine signaling and Th2 T-cell expansion by promoting JAK1 degradation via ITCH- and NEDD4L-mediated ubiquitination. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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