Thermo Fisher Scientific PPP1R1A Polyclonal Antibody
Human PPP1R1A 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며, 0.3 mg/mL 농도의 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PPP1R1A. Recombinant protein control fragment (Product #RP-102910). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645862 |
Product Specific Information
Immunogen sequence:
AESTETQESR PPGIPDTEVE SRLGTSGTAK KTAECIPKTH ERGSKEPSTK EPSTHIPPLD SKGANSV
Highest antigen sequence identity to the following orthologs:
- Mouse: 57%
- Rat: 60%
Target Information
Protein phosphatase inhibitor-1 (also designated inhibitor of protein phosphatase 1, IPP-1 and I-1) plays a role in regulating the phosphorylation of other proteins and is itself phosphorylated by a cyclic AMP-dependent protein kinase at Threonine 35.
Additionally, the proline-directed kinases Cdk1, Cdk5, and MAPK mediate in vitro phosphorylation of IPP-1 at Serine 67. In striatal tissues, glutamate-dependent regulation of NMDA-type channels influences IPP-1 phosphorylation at Ser 67.
The localization and expression of IPP-1 suggest discrete roles in specific brain regions and developmental stages, independent of protein phosphatase type 1 (PP-1) regulation. PP-1 binds both phosphorylated and dephosphorylated IPP-1, and conversion to a Mn++-dependent state appears to contribute to its regulation by IPP-1. IPP-1 attenuates glycogen phosphorylase activity and is thought to be important in hormonal control of glycogen metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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