Thermo Fisher Scientific BAG3 Polyclonal Antibody
Human BAG3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, TBS 버퍼에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 300 and 350 of human BCL2-associated athanogene 3 (BAG3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
BAG3 belongs to the BAG protein family, which competes with Hip for binding to the Hsc70/Hsp70 ATPase domain and promotes substrate release. All BAG proteins contain an approximately 45-amino acid BAG domain near the C-terminus but differ significantly in their N-terminal regions.
The BAG3 protein includes a WW domain in the N-terminal region and a BAG domain in the C-terminal region. BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain both in vitro and in mammalian cells, binding with high affinity and inhibiting chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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