
Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody
Thermo Fisher Scientific의 CCL5 (RANTES) Polyclonal Antibody는 고양이 단백질에 반응하는 sheep IgG 항체로, WB, ELISA, Neutralization에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 trehalose로 안정화된 동결건조 형태입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 0.2–0.8 µg/mL |
| Neutralization (Neu) | 0.5–2.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Cat |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant feline CCL5/RANTES Tyr26–Asn92 (Lys84Tyr and Gly85 deletion) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2606493 |
Product Specific Information
- In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human, mouse, and cotton rat RANTES is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).
Target Information
CCL5 (RANTES) is a chemokine gene located on chromosome 17q. Chemokines regulate immune and inflammatory responses and are categorized into four subfamilies based on cysteine arrangement.
CCL5 acts as a chemoattractant for monocytes, memory T helper cells, and eosinophils, induces histamine release from basophils, and activates eosinophils.
It is a major HIV-suppressive factor produced by CD8+ cells, functioning as a natural ligand for CCR5 and suppressing replication of HIV-1 R5 strains.
Alternative splicing produces multiple isoforms. Associated diseases include HIV Type 1 and Periapical Granuloma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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