Thermo Fisher Scientific LC3 B Polyclonal Antibody
LC3B 단백질 검출용 Rabbit Polyclonal 항체로 Western blot 및 IHC에 적합. 자가포식(autophagy) 연구에 활용 가능. Mouse 및 Rat 반응성. 동결건조 형태로 제공되며 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from mouse LC3 B conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
- The peptide is homologous in rat.
- Specificity: MAP1LC3B.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) is encoded by the gene MAP1LC3B in humans.
LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy—a process involving the bulk degradation of cytoplasmic components.
Three human LC3 isoforms undergo post-translational modifications during autophagy.
Macroautophagy, the major inducible pathway for cytoplasmic turnover, mediates degradation of cytoplasmic enzymes and organelles during nutrient starvation.
This process involves formation of double-membrane autophagosomes that fuse with lysosomes, releasing autophagic bodies for degradation.
LC3B mediates physical interactions between microtubules and cytoskeletal components and may play roles in cancer, aging, metabolic and neurodegenerative disorders, and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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