Thermo Fisher Scientific UBE2V2 Polyclonal Antibody
Thermo Fisher Scientific의 UBE2V2 Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가지며 Western blot, IHC, ELISA에 적합합니다. Rabbit IgG 폴리클로날 항체로, 고순도 친화 크로마토그래피로 정제되었습니다. DNA 복구 및 단백질 유비퀴틴화 연구에 활용됩니다.
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Thermo Fisher Scientific UBE2V2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–145 of human UBE2V2 (NP_003341.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.68 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806723 |
Product Specific Information
Immunogen sequence:
MAVSTGVKVP RNFRLLEELE EGQKGVGDGT VSWGLEDDED MTLTRWTGMI IGPPRTNYEN RIYSLKVECG PKYPEAPPSV RFVTKINMNG INNSSGMVDA RSIPVLAKWQ NSYSIKVVLQ ELRRLMMSKE NMKLPQPPEG QTYNN
Positive Samples: SW480, SKOV3, HeLa, HL-60, Mouse liver, Mouse skin, Rat liver, Rat brain
Target Information
The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1α,25-dihydroxyvitamin D3 (Vitamin D3). Using mRNA Differential Display technology, Fritsche J et al. identified a gene induced in freshly isolated human blood MO cultured for 4 hours with Vitamin D3. This cDNA encodes a 16.5 kD protein structurally homologous to yeast MMS2p and human CROC-1, belonging to the conserved Ubc-like protein family.
The protein, known as Ubiquitin E2 variant (UBE2V2) or MMS2, is required with Ubc13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These complexes are essential for assembling K63-linked polyubiquitin chains, acting as signals in DNA repair and IkBa kinase activation. Crystal structure analyses confirm the necessity of the Mms2/Ubc13 heterodimer in DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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