Thermo Fisher Scientific MHC Class II Monoclonal Antibody (HIS19), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except RT1n (including BN and MAXX strains).
RT1B, the rat MHC class II (I-A equivalent), is expressed by antigen-presenting cells such as B cells, dendritic cells, and macrophages.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of rat spleen or lymph node cells.
Recommended usage is ≤0.06 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells such as macrophages, dendritic cells, and B cells.
Before surface expression, MHC class II molecules bind to endocytosed exogenous antigens, which are then presented to T cells.
The antigen-binding groove between MHC class II alpha and beta chains is open at both ends and accommodates peptides 15–24 amino acids long.

HLA and MHC antibodies are critical in immunopeptidomics, aiding in the identification and characterization of neoantigens using high-performance liquid chromatography coupled with tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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