Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), APC, eBioscience
Mouse CD90.2 (Thy-1.2) 단백질을 인식하는 53-2.1 클론의 APC 결합 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. T 세포, 신경세포 등에서 CD90.2 발현 분석에 활용되며, 높은 특이성과 안정적인 형광 신호를 제공합니다.
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Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | View 55 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469421 |
Available Formats
- Unconjugated
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 555
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 760
- NovaFluor Red 710
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by thymocytes, mature T cells, and neurons in CD90.2-expressing mouse strains (e.g., BALB/c, CBA, C3H, C57BL/6, C58/, SJL).
Cells from CD90.1-expressing strains (PL, AKR) do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.06 µg/test (100 µL final volume).
Recommended careful titration for optimal assay performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein member of the Immunoglobulin superfamily.
It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
Thy-1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other brain events.
CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons, highly expressed in connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines.
In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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