Thermo Fisher Scientific p53 Monoclonal Antibody (OTI3D5), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 upregulates stress response genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory region.

Phosphorylation at Ser15 is essential for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a critical role in preventing proliferation of damaged cells and functions as an anticancer agent. It is also linked to aging, oxidative stress, and learning and memory processes. Mutations in TP53 are common in various cancers and cause loss of tumor suppressor activity. Germline mutations can lead to cancer-prone syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.