Thermo Fisher Scientific Moesin Recombinant Mouse Monoclonal Antibody (rMSN, 492)
Thermo Fisher Scientific의 Moesin 재조합 마우스 단클론 항체(rMSN, 492)는 인간 및 랫트 반응성을 가지며 Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. HEK293 세포에서 발현된 IgG1, kappa 형태로, 단백질 A/G로 정제된 액상 항체. 세포 부착 및 이동 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMSN, 492 |
| Immunogen | Recombinant full-length human Moesin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Moesin is a member of the talin-4.1 superfamily and acts as a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. Moesin is involved in cell adhesion, migration, and organization of cell surface structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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