Takara Bacterial expression and purification: pET express & purify kits

Overview

Cobalt-based resin—HisTALON

• No copurification of unwanted host proteins
• No SlyD contamination (histidine-rich protein present in E. coli)
• Lowest metal ion leakage
• Available in various formats as bulk resin, cartridges, and gravity columns

Nickel-based resin—His60 Ni Superflow Resin

• 60 mg/ml binding capacity
• Up to 95% purity
• Low metal ion leakage
• Available in various formats as bulk resin, cartridges, and gravity columns

More Information

Features

• Powerful—Higher inducible protein expression levels and tighter control of your target gene
• Fast—E. coli BL21(DE3)-based system
• Versatile—Choose between N- & C-terminal 6xHN-tagged vectors
• Convenient—Choice of fast, simple In-Fusion PCR cloning or traditional T4 DNA ligase cloning
• Complete—Purify your expressed protein with His60 Nickel resin or TALON Cobalt resin

Additional products

His60 Nickel resin is available separately as:

• His60 resins
• His60 gravity columns
• His60 FPLC cartridges

TALON Cobalt Resin is available separately as:

• TALON resins
• TALON gravity columns
• TALON FPLC cartridges

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.