Thermo Fisher Scientific Phospho-RUNX2 (Ser340) Polyclonal Antibody
RUNX2 단백질의 Ser340 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 사람, 생쥐, 랫트 반응성. PBS/glycerol buffer에 보관하며 -20°C에서 저장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RUNX2 (Accession Q13950), corresponding to amino acid residues around phosphorylated S340 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816682 |
Product Specific Information
This antibody detects endogenous levels of RUNX2 only when phosphorylated at S340.
Target Information
RUNX2 is a nuclear protein and a member of the RUNX family of transcription factors containing a Runt DNA-binding domain. It is essential for membranous and endochondral bone formation, regulating osteoblastic differentiation and skeletal morphogenesis. RUNX2 acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It binds DNA as a monomer or as a subunit of a heterodimeric complex and plays a key role in increasing TGFBR1 expression by osteoblasts. It cooperates with DLX5 or related factors to activate osteoblast-specific gene expression. Mutations in RUNX2 are associated with bone development disorders such as cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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