Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), FITC, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 1 µg/test
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Product Specifications

Additional Formats Available:

• APC
• Biotin
• Functional Grade
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T (especially TH1) and B cells, and its expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.

Signaling through SLAM in T cells induces proliferation and enhances the interferon-gamma response. It also plays a role in adhesion between T cells and antigen-presenting cells. The 9D1 clone is reported to be an activating antibody.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, the mShad150 clone (Product #12-1502) is recommended.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymic and splenic cells; ≤1 µg/test (100 µL final volume).
Cell number may range from 10⁵ to 10⁸ cells/test.
Careful titration is recommended for optimal assay performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation.
It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and some B cells, and is rapidly induced on naive T cells after activation.

Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.
SLAM-SLAM binding enhances expansion and differentiation of activated B cells.
CD150 also serves as the receptor for the measles virus and acts as a co-activator on T and B cells.
Interactions with SH2D1A and PTPN11 via its cytoplasmic domain have been reported, and mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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