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Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), FITC, eBioscience

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마우스 CD150(SLAM) 단백질을 인식하는 9D1 클론의 FITC 결합 단일클론 항체입니다. Flow Cytometry에 최적화되어 있으며, 1 µg/test로 사용 가능합니다. T세포 및 B세포 활성화 연구에 적합하며, 488 nm 레이저에서 사용 가능합니다.

카탈로그번호: 11-1501-82
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 11-1501-82 CD150 Monoclonal Antibody (9D1), FITC, eBioscience 100 ug pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), FITC, eBioscience

Applications

Flow Cytometry (Flow): Tested at 1 µg/test
View 6 publications

Product Specifications

항목	내용
Species Reactivity	Mouse
Published Species	Mouse
Host / Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	9D1
Conjugate	FITC
Excitation / Emission Max	498 / 517 nm
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE!
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_465209

Additional Formats Available:

APC
Biotin
Functional Grade
PE
PE-Cyanine7
Request custom conjugation

Product Specific Information

The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T (especially TH1) and B cells, and its expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.

Signaling through SLAM in T cells induces proliferation and enhances the interferon-gamma response. It also plays a role in adhesion between T cells and antigen-presenting cells. The 9D1 clone is reported to be an activating antibody.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, the mShad150 clone (Product #12-1502) is recommended.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymic and splenic cells; ≤1 µg/test (100 µL final volume).
Cell number may range from 10⁵ to 10⁸ cells/test.
Careful titration is recommended for optimal assay performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation.
It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and some B cells, and is rapidly induced on naive T cells after activation.

Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.
SLAM-SLAM binding enhances expansion and differentiation of activated B cells.
CD150 also serves as the receptor for the measles virus and acts as a co-activator on T and B cells.
Interactions with SH2D1A and PTPN11 via its cytoplasmic domain have been reported, and mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.