Thermo Fisher Scientific C4 binding protein Polyclonal Antibody
Thermo Fisher Scientific의 C4 binding protein Polyclonal Antibody는 인간 및 랫트 반응성을 가진 Rabbit IgG 항체입니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합하며, KLH 결합 합성 펩타이드로 면역화되었습니다. 고순도 Protein A 정제 및 안정적인 보관 조건 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human C4 binding protein, amino acids 1–100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Complement component 4-binding protein (C4BP) is a plasma glycoprotein that inhibits the classical pathway of complement activation, which is mediated through antibody targeting of foreign antigen. Structurally, C4BP is a disulfide-linked, multimeric protein composed of seven alpha chains and one beta chain. It functions as a cofactor for C3beta inactivator in the cleavage of C3beta and accelerates the decay of C4betaC2alpha (C3 convertase) by acting as a cofactor in the cleavage of C4beta by factor I.
Streptococcal strains expressing Ig-binding surface molecules (M protein family) can bind overlapping C4beta binding sites in C4BP, interfering with the classical complement activation pathway. Bacteria-bound C4BP may represent an evolved mechanism to downregulate complement activation in the bacterial microenvironment, reducing opsonization and phagocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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