Thermo Fisher Scientific CDK2 Monoclonal Antibody (OTI4B4), TrueMAB
CDK2 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow cytometry에 적합. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. Affinity chromatography로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B4 |
| Immunogen | Full length human recombinant protein of human CDK2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed with CyclinO. CyclinO, a specific isoform of the UNG gene, is localized in the nucleus and exhibits uracil-DNA glycosylase activity.
CDK2/cyclinO protein levels increase during the G1 phase and are degraded as the cell cycle progresses. The cyclin A–cdk2 complex peaks at the G1/S transition and associates with DNA during replication initiation. Rb protein serves as a substrate for cdk2–cyclin E and/or cdk2–cyclin A in vivo. Cdk2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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