Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), FITC, eBioscience
인간 CD45 항원을 인식하는 HI30 클론 단일클론 항체로, FITC로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 모든 백혈구 계통 세포 표면의 CD45 발현 검출에 사용됩니다. 4°C 암소 보관, 동결 금지.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 publications |
| Immunohistochemistry (IHC) | - | 5 publications |
| Immunocytochemistry (ICC/IF) | - | 6 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | 58 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI30 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10854279 |
Additional Available Formats
Unconjugated, Alexa Fluor 532, 561, 647, 660, APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–711), eFluor 450/506, NovaFluor Blue/Red/Yellow series, PE, PE-Cyanine series, PerCP series, and custom conjugation available.
Product Specific Information
The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase activity and plays an important role in lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
- Recommended Usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Excitation/Emission: 488 nm / 520 nm (Blue Laser)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (Leukocyte Common Antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins. It is absent in non-hematopoietic cells and tissues. CD45 regulates Src-family kinases, lymphocyte development, and antigen receptor signaling. Multiple isoforms arise from alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 plays essential roles in T- and B-cell activation, cytokine receptor signaling, and integrin-mediated signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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