Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), FITC, eBioscience
마우스 CD45.2 항원을 인식하는 단클론 항체로, FITC 형광 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 다양한 마우스 균주에서 백혈구 표지에 사용됩니다. 고순도 친화 크로마토그래피 정제, 4°C 보관, 연구용 전용입니다.
- 카탈로그번호
- 11-0454-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 137 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465060 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711 / 786
- eFluor 450 / 506
- NovaFluor Blue 510 / 555 / 585 / 610-70S / 660-120S
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation
Product Specific Information
Description:
The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.
Excitation/Emission: 488 nm / 520 nm (Blue Laser)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising approximately 10% of lymphocyte surface proteins. It is crucial in lymphocyte development and antigen signaling, regulating Src-family kinases.
CD45.2 exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. These isoforms vary in membrane domain localization and expression depending on cell type and physiological state.
Beyond immunoreceptor signaling, CD45.2 promotes cell survival via integrin-mediated pathways and participates in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex with restricted expression among lymphoid subtypes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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