Thermo Fisher Scientific HNF1A Monoclonal Antibody (7H3)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

7H3

Immunogen

Fusion protein corresponding to residues 109-269 of human TCF1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2116750

Product Specific Information

Recommended positive controls: Jurkat.

MA1-25417 is expected to cross react with mouse (100% conserved), rat (100% conserved), Xenopus laevis (86% conserved), chicken (99% conserved), and hamster (99% conserved) due to sequence homology.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.