Thermo Fisher Scientific HNF1A Monoclonal Antibody (7H3)
HNF1A 단백질을 인식하는 mouse monoclonal antibody(7H3)로 Western blot, IHC(F), IP에 사용 가능. 다양한 종과 교차 반응성이 높으며 Jurkat 세포를 양성 대조로 권장. 연구용으로 RNA polymerase II 전사 조절 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7H3 |
| Immunogen | Fusion protein corresponding to residues 109–269 of human TCF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2116750 |
Product Specific Information
- Recommended positive control: Jurkat
- MA1-25417 is expected to cross-react with mouse (100% conserved), rat (100% conserved), Xenopus laevis (86% conserved), chicken (99% conserved), and hamster (99% conserved) due to sequence homology.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region and may play a role in the phenotype of WHS or Pitt-Rogers-Danks syndrome.
The encoded protein can react with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a potential target for specific immunotherapy in cancer research. It is also a member of the NELF (negative elongation factor) complex involved in RNA polymerase II transcription elongation regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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