Invitrogen™

pcDNA™5/FRT/TO Vector Kit

pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid,자세히 알아보기

pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.

The pcDNA™5⁄FRT⁄TO vector contains the following elements:

• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells
• Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line
• Hygromycin resistance gene for selection of stable cell lines

사양

구성 또는 유도성 시스템Inducible

배달 유형Transfection

유도제Tetracycline

프로모터CMV/TO

제품 유형Flp-In/T-REx System Expression Vector

선택 제제(진핵)Hygromycin

단백질 태그Untagged

클로닝 방법Restriction Enzyme/MCS

수량20 μg

벡터pcDNA

제품라인Flp-In™, T-REx™, pcDNA™

용도(애플리케이션)Regulated Expression

Unit Size20 µg