Thermo Fisher Scientific ENC-2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 360 and 589 of Human KLHL25 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ENC-2,uniProtId:Q9H0H3-1,ncbiNodeId:9606,antigenRange:360-589,antigenLength:589,antigenImageFileName:PA5-31115_ENC-2_Q9H0H3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31115_ENC-2_Q9H0H3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.69 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548589

Product Specific Information

Recommended positive controls: NT2D1.

Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (92%), Rhesus Monkey (99%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

KLHL25 (ectoderm-neural cortex protein 2, ENC2) is a cytoplasmic protein that contains six Kelch regions and a single BTB (POZ) domain. KLHL25 is highly homologus to another Kelch-like protein, ENC1, and it is believed to operate in a manner similar to other Kelch-domain containing proteins. Kelch-domain repeat containing proteins often act as modifiers of Actin fibers. Expressed early in embryogenesis, ENC1 helps to mediate neuronal process formation. It also appears to have a role in neural crest cell differentiation. KLHL25 likely functions as a substrate specific adapter for protein ubiquitinating complexes. KLHL25 is expressed in most tissues with highest expression in brain and liver.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.