Thermo Fisher Scientific RAP1GAP Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive Control: mouse brain, rat brain
• Predicted Reactivity: Mouse (100%), Rat (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1.
Physical interaction between G alpha z (a member of the Gi family of trimeric G proteins) and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase.

Rap1GAP is expressed in the brain, kidney, and pancreas, and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rap1 GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.