Thermo Fisher Scientific TGF beta-1 Monoclonal Antibody (2C5)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) forms of TGF beta under both non-reducing and reducing conditions.

Target Information

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-β is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of αV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-β binding proteins.

Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor (TGF-LAP), and a specific binding protein (LTBP). TGF beta-1 regulates proliferation, differentiation, adhesion, and migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors.

TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.