Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), FITC, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.25 µg)/test
  • Publications: View 1 publication

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin gene superfamily. The N-terminal region (aa1–107) is homologous to Ig kappa light chains, while the C-terminal extracellular domain is proline-rich and forms a stalk structure.
CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, a subset of normal B cells, and malignant B cells. Cross-linking of CD7 modulates T and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 associates with phosphoinositol 3′-kinase, and ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.

A subpopulation of human CD34(+) CD38(-) cord blood cells expressing high levels of CD7 has lymphopoietic potential, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid lineages. The CD7(+) phenotype distinguishes primitive lymphoid progenitors from pluripotent stem cells.
CD7 may also cooperate with CD28 in Treg function, as double-deficient mice show reduced Treg numbers and suppressive activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.25 µg) per test (defined as the amount required to stain a 100 µL cell sample).
Typical cell number: 10⁵–10⁸ cells/test.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. Its N-terminal domain (aa1–107) is homologous to Ig kappa light chains, while the C-terminal region is proline-rich. CD7 is found on most immature and mature T lymphocytes, T cell leukemias, NK cells, a subset of normal B cells, and malignant B cells.
Cross-linking of surface CD7 modulates T and NK cell activity, enhancing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3′-kinase, and ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.
CD7 expression is an important marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지