Thermo Fisher Scientific Calsequestrin Polyclonal Antibody
Rabbit polyclonal antibody targeting Human Calsequestrin-1/CASQ1. Suitable for WB, IHC, ELISA, and IP applications. Preservative-free formulation with high specificity for calcium-binding protein in muscle SR. Ideal for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000–1:20,000 |
| ELISA | 1:25,000–1:50,000 |
| Immunoprecipitation (IP) | 1–2 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Calsequestrin-1/CASQ1 Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Lot-Specific |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 1 month. For long-term storage, store at –20°C. |
| Shipping Conditions | Wet ice |
| RRID | AB_2787728 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human Calsequestrin-1/CASQ1.
Target Information
The sarcoplasmic reticulum (SR) helps regulate intracellular calcium levels in cardiac and skeletal muscle by storing and releasing calcium. Among several SR calcium-binding proteins, calsequestrin is the most prominent.
Calsequestrin sequesters calcium accumulated in the SR during muscle relaxation and is localized to the junctional and corbular (terminal cisternae) regions. It functions to localize calcium near the junctional face of the terminal cisternae, from which calcium can be released into the cytosol via the ryanodine receptor.
This protein is highly acidic, with a large capacity and moderate to low affinity for calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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